The Lightning Project

The ongoing saga of the PNG Lightning Maroon Clownfish breeding project.

Somewhere along the line I alluded to the fact that there was at least one US-based person involved in the Lightning Maroon auctions who had the foresight to obtain wild PNG Maroon Clownfish from Scott Fellman at Unique Corals with the express intent of  using them as mates for the offspring from this project.  I tried to do the same, but as I’ve shared before, the abrupt loss of exports from EcoAquariums PNG meant that once again, I was thwarted in my own attempts to beef up my base of wild genetics.

A wild (F0) Premnas biaculeatus "PNG White Stripe" Maroon Clownfish purchased through Unique Corals.

A wild (F0) Premnas biaculeatus “PNG White Stripe” Maroon Clownfish purchased through Unique Corals.


That person I alluded to is Brandon Mehlhoff.
Lightning Maroon fans, meet Brandon Mehlhoff.

Lightning Maroon fans, meet Brandon Mehlhoff.


I won’t share all the details, but we sat across the table from each other at the post MBI Workshop dinner this year and the topic of PNG Maroons came up, and the fact that he had one (the fish shown above).  I basically said “name your price”, at which point he revealed who he was and why the fish was not for sale, at any price.  The moment I learned of his intentions, my thought shifted completely from “I gotta get him to part with this fish” to “this guy really needs a Lightning Maroon, as his outcross pairing of a F1 from my breeding to a F0 from PNG, will help start a distinct line and expand the genetic base for the entire captive Lightning Maroon population”.
Lucky for Brandon, he got “the fish” he needed in round 2 of the auctions, the winning bidder of LM15.
Lightning Maroon #15 (LM15) - Brandon's fish.

Lightning Maroon #15 (LM15) – Brandon’s fish.


While most fish were destined to be shipped out, Brandon proved his dedication to the project by requesting that he personally pick up and transport his fish.  Brandon, a North Dakota native and rare clownfish enthusiast (he also maintains Mccullochis among others) made a 24 hour whirlwind trip to personally come to my fishroom.
Of course, it was a great excuse to get some much needed cleanup done (still an ongoing process as I’m setting up more growout for FW in my formerly open space in the middle of the room…I now have two aisle).  Brandon got to see his fish, and all the others firsthand.  Of course, he was able to pay his respects to “Mama Lightning”.
Brandon Mehlhoff did what any good aquarist would do...

Brandon Mehlhoff did what any good aquarist would do…


 
..asked if he could take photos....

..asked if he could take photos….


 
...and took lots of them!

…and took lots of them!


I certainly would’ve let Brandon stay all day if he liked, but he still had to drive home and put away some very precious cargo.  Time to bag ’em up:
Very rare you get to see ME in the photos, here netting LM5 for the journey to Brandon's fishroom.

Very rare you get to see ME in the photos, here netting LM5 for the journey to Brandon’s fishroom.


Bagging up Lightning.

Bagging up Lightning.


After a long drive home and acclimation into the wee hours of the morning, Brandon was very happy to send me a few photographs of LM15 in the new fishroom.  This pairing is going to be an important one owing to the outcrossed genetics.  Depending on who’s interpretation of filials you care to go by, Brandon’s potential offspring are either F1 or F2 (from a more generalized viewpoint, they are F1 in the greater scientific community.  In the aquarium world, they *might* be called F2 by many since one parent is F1).
Either way, an important pair to follow in terms of genetic stability and known provenance for Lightning Maroon Clownfish.
Brandon Mehlhoff's Future F1 Lightning X F0 PNG Maroon pairing

Brandon Mehlhoff’s Future F1 Lightning X F0 PNG Maroon pairing


 
 

The forced size differentiation of my holdback Lightning pair continues and is going well…my chosen female is growing larger and larger.  It’s “only a matter of time”.












 

So I was gone for only 24 hours….24 HOURS, and I come back to find that the fry from Spawn #8 are gone.  Not sick, not dying, not dead.  GONE.  As in VANISHED.  As in “NO BODIES”.  Honestly I have no clue what’s going on there…it was a small group, probably less than 10, with several lightnings showing.  Realistically, I’m pretty sure something happened, they died, and their tiny corpses rotted away before I even had a chance to notice.
And yet, here we go again – I returned home on 8-26-2013 to find a freshly-laid nest, spawn #10. Looks like September 2nd will be my “pull night” for the larvae, lest I have another failure like I did with Spawn #9. Interestingly, the pair has changed which tile they spawn on.  Wish us all luck!





 

What’s it going to take to get a good batch of Lightning Maroons to hatch out?  Seriously!
So you’ll probably know that I’ve been having problems both in timing the pulling of the nest, as well as split hatches and/or eggs dying if pulled prematurely.  While it seems that eggs are often ready to hatch on the 7th night, historically the bulk hatch out on the 8th night.  Even with the last spawn, #8, 8 days to pull was the right number by my estimates.  A side note on spawn #8 – they underwent metamorphosis while I was away at my brother’s wedding (got the text from my good friend Mike Doty)…looks like we might have maybe 8-10 offspring growing up.  So in total, maybe 10 F1 Lightning Maroon offspring that could be released in 2014.
Back to spawn #9 – the night of August 20th, I looked at the huge viable nest from spawn #9.  Spawn #9 was laid on 8-13-2013.  By all accounts, the night to pull this nest for hatching was not 8-20-2013, but the evening of 8-21-2013. I left the eggs in the tank on 8-20-2013.  Just in case, I left ON a few extra lights in the room to help prevent a premature hatch…or so I thought.
Imagine my frustration when I went into the fishroom yesterday morning, 8-21-2013, and saw a clean tile with no eggs anywhere to be found.  That’s only 7 nights, with a 100% hatch!  I’m past the point of getting overly upset about such mishaps, primarily because the Lightning Maroon already appears to be ready to put down another nest.  Perhaps not being blinded by rage and frustration allow me to make an observation.  I left a few more ambient lights on than normal, did not move the nest, and got a 100% hatch all on one night – the first time I’ve not documented a split hatch over two nights.
Could it be that slightly elevated light levels replicate a “full moon” scenario (combined with the fact that we’re basically at a full moon right now) and encouraged a full and complete hatch vs. the split hatch?  In talking with other clownfish breeders, it seems that the “split hatch” tends to be a problem they wind up just “living with” and more than one seems to have this problem.  Perhaps we’re taking the wrong approach towards light on hatch night?

The Lightning Project - Lightning Maroon Clownfish on Facebook
Yes, I finally took the time to set up the page.  So go “like” it!
https://www.facebook.com/lightningmaroonclownfish
Last fish of the official 2nd round of Lightning Maroon Clownfish auctions hits eBay tonight!

LM16 – the first offspring to be auctioned in this second round of releases,


Time to start watching eBay again; be sure to add Blue Zoo Aquatics to your list of favorite sellers!  From the latest auctions:

“…it is now time to release for sale the next 6 Lightning Maroon Clownfish. After nearly 3 years of toil, trouble and extreme dedication, breeder Matt Pedersen is proud to offer to the US market the next batch of these truly remarkable fish. For those discovering this amazing animal for the first time, please click HERE to familiarize yourself with the extraordinary journey that has taken place to bring these fish to market.
These next 6 will be auctioned off in individual eBay listings. Each listing will be staggered by about 12 hours to give everybody the best chance to obtain one of these remarkable fish. Four of these fish display the striking lightning pattern similar to their mother while two of these fish look similar to a normal patterned maroon clownfish except for distinctive jagged striping and the odd white dot in different places. These two siblings are the only PNG bloodline maroon clownfish on the market and the best bet for continued breeding and development of the lightning pattern. Matt has more information about this on his website, http://www.lightning-maroon-clownfish.com so be sure to check it out.”

As I type this, the first two fish have already been started.  Newly introduced LM15 and LM16 are already up; the bidding started at $0.99 as it has with all our fish so far!  2 more Lightnings, and 2 White Stripe siblings, yet to come! Remember, you can quickly find all the live auctions here on the site (upper right hand right rail) or by visiting Blue Zoo’s eBay store.

LM15 – also up for auction now. 4 more fish to come!

Just a quick note this evening.
First, anyone care to guess what it means when this shows up in Duluth, MN?

Blue Zoo Aquatics Boxes...lots of them...

Blue Zoo Aquatics Boxes…lots of them…


The big news though is some changes to the inventory page of offspring.  In the past couple weeks I’ve added in some of the offspring that I gave away, and I’ll get the other donated pairs in there soon.  I’ll also be adding in my own personal holdbacks soon too; the ultimate goal being a more complete accounting of ALL the offspring.

One fish, a white stripe offspring, WS5, has changed in status and will not be sold at auction because it was hand delivered as a replacement for a fish lost in a brownout before delivery.  My apologies to anyone who had their hearts set on WS5, but there are still many other White Stripes to be offered.
While we’re a white stripe short (and I might need to pull another one from inventory to make my last planned White Stripe X Lightning pair donation), two new Lightning Maroons have been added to the “for sale” inventory tonight!  These were fish I had in the holdback cubes; they are now considered fish that will be offered for sale.
Introducing…
Lightning Maroons #LM15 and #LM16

Newly added to the for-sale list – Lightning Maroon #15 (LM15)


Also now up for grabs, the newly released Lightning Maroon Clownfish #16 (LM16)


 

So yes, there was a spawn I posted a photo of back on July 31st; spawn #8; this was the one they put down on July 29th. I didn’t run the larval snagger on the night of August 5th as I had hoped, because I still don’t have mine back from it being lent out at least a year prior…not that I even had TIME to go ask for it! Unfortunately, come the evening of August 6th, it was quite clear that MOST of the eggs had hatched, and come night time, I was left with maybe a quarter-sized nest from what had started as several square inches! I pulled the nest for hatching on the 6th, and come morning on the 7th, I had only a handful of larvae.  MOST of the eggs did not hatch, and died later in the day.
Now, 7 days later, I seem to have the same 20 or so offspring that hatched out.  I only added RotiGreen Omega once, right at the start, and only now am seeing things clear up to the point where I might have to do another dose.  Rotifers here lately have been weak following the simultaneous crash of 2 of my 3 cultures, and they haven’t really been rebuilding.  Thus, I’ve never established a self-sustaining population of rotifers in the larval tub, and instead have had to resort to daily influxes.  Still, things are going well I suppose, and today I added in the first offerings of TDO size A.
The good news is that the pair certainly seems to be in a routine, as this afternoon, 8-13-2013, they put down another sizable nest.  This is spawn #9 from the pair. It’s only a matter of time before I figure out how to get a BIG group of offspring from these eggs instead of a piddly amount, but until then, it’s going to continue to be a slow trickle at best.

After the first round of auctions with Blue Zoo Aquatics, we came to the mutual conclusion that sticking with our original plan, one of drop shipping the fish from here in Duluth to the final recipients, is going to be our preferred route going forward. I had so many direct inquiries asking why I had forced the fish to go through an extra trip to the west coast, and in truth, neither Mark nor myself had originally planned that.  Quite simply, with a brand new daughter who has a penchant for screaming constantly and uncontrollably, it felt as if the only way we’d ever get the ball rolling on sales was to have me make one shipment to Blue Zoo.  It worked, but public sentiment was definitely against the extra trip.
With our decision to stick with drop shipping from Duluth, this means I need to hold, and segregate, most all of the maroons here to ensure that they don’t turn on each other in a murderous rage as fish are removed from the community. This means that all of the Maroons in my holding cubes, the ones that were destined to be distributed as backup pairs, held back for test pairings and breedings here, as well as smaller specimens, all need to be moved out to permit me to segregate the remaining 25 or so fish that we’ll be selling. I’m running out of space.
In an effort to free up holding cubes, I’m pushing to place and pair many of the fish I’ve been holding in segregation. As you’ve probably read in the past, I’ve planned several backup pairs to be spread around the city and country, just in case anything were to ever happen to our home or fishroom. So, this Friday, August 2nd, 2013, another one of those pairs was selected from my holdbacks and placed under the care of the staff at the Great Lakes Aquarium (GLA), our local public aquarium situated on the shores of the Duluth Harbor / St. Louis Bay, Lake Superior.

Great Lakes Aquarium in Duluth, Minnesota. Lake freighter Canadian Transport passing Duluth Aerial Lift Bridge : Photo by Tmajewski.

Great Lakes Aquarium in Duluth, Minnesota. Lake freighter Canadian Transport passing Duluth Aerial Lift Bridge in the background : Photo by Tmajewski, under Public Domain license (THANKS Tmajewksi!)


The Great Lakes Aquarium is mainly a freshwater aquarium (all “permanent” exhibits are freshwater) as the mission is primarily to educate visitors about the waters in our region. As such, it’s fair to say that when we first moved to Duluth, the marine hobbyists I knew didn’t exactly give it a “must see” rating.  I think it took a year and a half before we ever stepped foot in our own public aquarium.
Since that time, I’ve come to grow fond of our local aquarium. The facility is wonderful, the building is beautiful. With a family membership, we can escape there for an hour or a day, and it’s very kid friendly. Ethan loves it (the water table, the treehouse slide, and the bear den are his perenial favorites).
Even though the GLA has suffered budget setbacks and more than one oversight change since it opened in 2000, it’s worth the visit if you’re here. I can only say things continue to improve and yes, more and more saltwater displays are making their way into the aquarium.  Like it or not, I think everyone understands that “Nemo”, “Seahorses” and Otters are a likely larger draw than Largemouth Bass and Rainbow Trout; while the mission is most admirable, it’s difficult to accomplish if people aren’t lining up to see the same fish they caught when fishing the day before.
Ultimately, I look at the Great Lakes Aquarium and see an organization that is growing, refining, and only improving, something I can be ever more proud of to call my home aquarium and an institution that our city should be very proud of and continue to prioritize. Heck, with Sepia bandensis on display from none other than Rich Ross at the Steinhart, and a fledgling new coral reef aquarium as part of the Fire, Ice, and the Rise of Life (see Lake Superior Magazine | Great Lakes Aquarium website) introduction to the history of our region (which happens to include a new Chambered Nautilus exhibit), offerings that will draw the layperson in are on the uptick. There’s plenty of massive envy-inducing Brook Trout still on display, the likes of which I’ll never catch in the wild!
Another view of the Great Lakes Aquarium, now home of a Lightning Maroon Clownfish - Image by Randen Pederson under Create Commons License (thanks Randen!)

Another view of the Great Lakes Aquarium, now home of a Lightning Maroon Clownfish – Image by Randen Pederson under Create Commons License (thanks Randen!)


So when it came time to think about a public aquarium where I could place a Lightning Maroon for public viewing, I certainly had options.  I grew up with the Shedd and still have acquaintances who work there.  No doubt, many of my other friends who work at and run public aquariums would have welcomed the contribution, east coast or west, north or south. Ultimately though, my humble home of Duluth, Minnesota, is where the Lightning Project took place.  It’s where a single wild variant spawns and tends here eggs, and where her progeny have grown up and in turn been sent back into the aquarium hobby. As much as the Lightning Project is a PNG story, a SEASMART story, a Pacific Aquafarms story, a Blue Zoo Aquatics story, or a Matt Pedersen story, it is also now a Duluth story.
We’ll probably never have a MACNA in Duluth, MN.  Heck, we’re lucky to get 10 people to a meeting of the Lake Superior Marine Aquarium Club (LSMAC). For a small city that’s welcomed me and my family with kindness and support, it is only fitting that I give back to the home I hope I never have to leave. There really was only one choice, and having had the help of folks like aquarium staffers Barb and Heidi earlier in this project (they actually were here and witnessed the spawning of the first successful offspring, the fish they are now holding), the Great Lakes Aquarium truly was the only and best choice. I’m really glad they accepted my offer.
The details of my donation to the aquarium are simple.  The fish were provided freely (obviously) and the only stipulations were these. 1st, if the aquarium for some reason could no longer house the fish, that they be returned to me, and 2nd, the eggs are mine if I need them.  In other words, they are a backup pair for my fishroom and another avenue of genetic diversity to help keep mutliple F1 pairs out there in production, which in turn keeps the genetics diverse in the greater population.  The more F1 fish we have producing F2 fish, the better we are from a big-picture standpoint.
The Fish Donated to the Great Lakes Aquarium
In short, I hand delivered 1 Lightning Maroon, and one White Stripe Maroon sibling, to the Great Lakes Aquarium this afternoon.  I’ve added to them to the offspring catalog and assigned them the ID’s GL1 and GL2 (I’m sure someone, somewhere, will name these fish more fitting names!)  Here’s their photos (and links to their individual pages)
GL1 - a F1 PNG Lightning Maroon Clownfish donated to the Great Lakes Aquarium in Duluth, MN, USA

GL1 – a F1 PNG Lightning Maroon Clownfish donated to the Great Lakes Aquarium in Duluth, MN, USA


MORE on Lightning Maroon GL1
GL2 - a F1 PNG White Stripe Maroon Clownfish donated to the Great Lakes Aquarium in Duluth, MN, USA

GL2 – a F1 PNG White Stripe Maroon Clownfish donated to the Great Lakes Aquarium in Duluth, MN, USA


MORE on White Stripe Maroon GL2
How to strike the Great Lakes Aquarium with Lightning
The actual mechanics of ensuring the Great Lakes Aquarium was the recipient of a Lightning strike were fairly mundane.  Fish were bagged…
GL2, reading for bagging

GL2, reading for bagging


GL1, also reading for bagging


Even though it's a 15 minute drive, the fish are bagged with oxygen and placed in a shipping styro.  Nothing but the best...

Even though it’s a 15 minute drive, the fish are bagged with oxygen and placed in a shipping styro. Nothing but the best…


…and simply driven to the aquarium.  Just about as stress free a trip as they could ask for.
I gotta say, I kinda felt like Santa once I got there. I have never seen gossip travel so fast through a workplace; it seems like anyone who COULD get away and sneak a peek, did just that!
GL1 and GL2 floating for acclimation in the behind-the-scenes quarantine tank where they will be conditioned and paired before going on display.

GL1 and GL2 floating for acclimation in the behind-the-scenes quarantine tank where they will be conditioned and paired before going on display.


Of course, I think Jadell (will withhold her last name for privacy) was both the happiest and most stressed, as I believe it is she is the one who manages the marine exhibits (including the fantastic Seahorse breeding) and has been put in charge of the Aquarium’s Lightning Maroon and mate.  She hides her nervous terror well behind that beaming smile. Don’t worry Jadell, I know that feeling; I knew it when their parents first showed up here.
Jadell, you'll do great, I know you will!

Jadell, you’ll do great, I know you will!  And no worries, there are more if you need ’em.


No word on when we’ll have these fish paired and ready for display, but I think it’s going to take 3 to 6 months before a solid pairing can be created using segregation and forced size differentiation to ensure that the Lightning Maroon grows faster and larger than the White Stripe sibling.
When ready, Jadell has told me that this middle aquarium is due to be the new, revamped home for the pair.

The aquarium in the center is the currently planned new display home for the Lightning Maroon and her mate, when the time comes.


You can be sure that I’ll continue to watch this pair and post an update when there’s some news!  You may never get to come into my house to see the original Lightning Maroon, but for the price of admission, someday soon you’ll be able to see one in person at the Great Lakes Aquarium.

Two photos shot tonight in the fishroom – one is the pair on their latest spawn, and the other is my personal favorite holdback, the one I’m using to document pattern development 😉  Enjoy!

Lightning Maroon Clownfish and it's White Stripe Mate, tending to their 8th spawn.

Lightning Maroon Clownfish and it’s White Stripe Mate, tending to their 8th spawn.


 

Now over a year old, the pattern is still developing on all these clownfish!